Inoculation of Deinococcus Radiodurans for Future 7kb Plasmid Research
Introduction Last semester in lab, a plasmid isolation was done on Deinococcus Aquaticus. when this plasmid was run on a gel, a 7kb strand was visible. No information was known about this mystery plasmid. Since then, our sample has denatured or was discarded accordingly. This semester, we (Aland Ali, Anh Nguyen, Rhiannon Giesegh, Mike Agneskiy) plan on farming and growing this plasmid to discover more of its characteristics. However, this week mainly consisted of a “bootcamp” of sorts that provided a refresher course of lab protocols, safety, storage, hygiene, and overall etiquette. Due to this, not much time was spent on beginning our projects until the end of the week. This time was spent creating TGY Agar plates and inoculating Deinococcus Aquaticus from freeze bag to farm our future plasmid. Procedure To begin, 8 TGY Agar plates were created, labeled, and left overnight to ensure proper gel-like consistency. Next, Deinococcus Aquaticus from a freeze bag was acquired and set in a